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Thermo 反转录试剂盒/Thermo First Strand cDNA Synthesis Kit
货号：Q1010 规格：20T 价格：500
货号：Q1010L 规格：50T 价格：1050
产品简介
本制品包含cDNA第一链合成所需要的全部试剂，其中的反转录酶可以利用oligo(dT)18 primer/Random primer/基因特异性引物等进行反转录反应，合成的第一链cDNA可广泛用于cDNA第二链的合成、杂交、PCR扩增、Real-Time PCR反应等。该反转录酶是对M-MLV进行定点突变修饰后的新型反转录酶，具有较弱的RNAseH 活性和较高的热稳定性，酶活性高，反转录效率快。可在42℃－60℃之间合成较长的cDNA。试剂盒中的RNAase inhibitor对RNAase表现高度的非竞争性抑制，从而降低RNAase对RNA的影响，利于反转录反应的进行。本试剂盒同时优化了反应Buffer，能使反应的模板量（Total RNA）可在0.1-5 μg范围之间进行逆转录反应。cDNA合成后进行PCR扩增既可检测到长cDNA（>10 kb）片段，也可检测到低丰度cDNA样品。
产品储存条件
1. -20℃，一年有效；
发表文献精选：
[1] IF=2.323 Ovarian Circular RNAs Associated with High and Low
Fertility in Large White Sows during the Follicular
and Luteal Phases of the Estrous Cycle;Animals 2020, 10(4), 696
[2] IF=2.985 A zebrafish (danio rerio) model for high-throughput screening food
and drugs with uric acid-lowering activity; Biochemical and Biophysical Research Communications 508 (2019) 494e498
[3] IF=3.191 Genome-Wide DNA Methylation Profile
of Gene cis-Acting Element Methylations
in All-trans Retinoic Acid-Induced Mouse Cleft Palate; DNA AND CELL BIOLOGY
Volume 37, Number 12, 2018
[4] IF=2.274 Analysis of long non-coding RNA expression profiles following focal cerebral
ischemia in mice; Neuroscience Letters 665 (2018) 123–129
[5] IF=2.773 Accumulating mechanism of γ-aminobutyric acid in soybean
(Glycine max L.) during germination; International Journal of Food Science and Technology 2018, 53, 106–111
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